Green Synthesis of Copper Oxide Nanoparticles using Microtrichia Perotitii Dc Plant Extract: Characterization and Antibacterial Activity

Abstract

This paper describes the successful manufacture of copper oxidenanoparticles using leaf extracts from the Microtrichia perotitii DC plant as a chelating agent and copper tetraoxosulphate (vi) pentahydrate (CuSO₄.5H₂O) as a precursor. The nanoparticles were thoroughly characterized by UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray analysis combined with scanning electron microscopy, and thermogravimetric analysis. X-ray diffraction examination revealed the formation of CuO NPs with an average crystallite size of 64 nm. Scanning electron microscopy pictures revealed a primarily heterogeneous sample with a combination of flaky and irregular particle morphologies, indicating some degree of nanoparticle aggregation. Fourier transform infrared spectrophotometer examination revealed a strong bond at 647 cm^-1, which corresponds to the stretching vibration mode of CuO nanoparticles. Thermogravimetric studies indicated that the nanoparticles are thermally stable. The synthesized nanoparticles showed antibacterial efficacy against B. subtilis, with a zone of inhibition measuring 21.875±3.22 mm