Bacterial Bio-detector for CdCl2 and NiCl2 Heavy Metal Pollutants Based on Their Optical Properties

Abstract

In fundamental neuroscience and cell biology, gadgets for studying living cells have been of great interest. Recent research has expanded cell-based sensors to pharmacological screening, environmental monitoring, and toxicological detection. Among the many measurement methods, optical sensors make bioactivity and cell population analysis easy. This research is part of a larger effort to develop microorganism-based heavy metal (toxin) sensing methods. A correlation was established to examine how heavy metals affect E. Coli and Deinococcus radiodurans bacteria's optical density. CdCl2 and NiCl2 were used for this study. In this study, Escherichia coli (E. coli) and Deinococcus radiodurans (D. radiodurans) were exposed to heavy metal solutions. Dissolving the compounds in de-ionized water generated 0.1, 1, 10, 100, and 1 M CdCl2 and NiCl2. This study used fluorescence microscopy, spectrophotometers, and fluorescence spectroscopy.