Preparation of Fe2O3 Nanoparticles from Mixing Henna Extract With Ferric Chloride to Cytotoxic Assay on Cancer Cell Line

Abstract

Green synthesis of cytotoxic assay of iron oxide nanoparticles (Fe₂O₃) NPs was on cancer cell line prepared from mixing (henna) extract with iron chloride (III) salts at 200 ^oC for 2 hours by simple chemical method. (Fe₂O₃) NPs were identified using X-ray diffraction, filed Emission-Scanning Electron Microscopy, Ultravolite visble, and Photoluminescence. XRD measurements explained crystalline size (30) nm with (hexagonal) structure for (Fe₂O₃) NPs using henna extract. FE-SEM showed average grain size of (Fe₂O₃) NPs were (18.61) nm. (Fe₂O₃) NPs were tested for their cytotoxic effect against human cancer cells and inhibition rate % results were very high. Results of inhibition rate % for (Fe₂O₃) NPs using henna extract for human cancer cells were (78.9%).