Silver Nanoparticles Synthesis Using Chitosan Extract by a Simple Chemical Method and Application in Bntibacterial Activity

Abstract

Ag nanoparticles (Ag NPs) were prepared via simple chemical method at 200 ^oC for 2 hours using chitosan extract and sliver (iii) nitrate (Ag₂NO₃) salt. The chitosan extract acts as a reducing, stabling and anti-caking agent to transfer Ag₂NO₃ salt to Ag NPs in short period of time. Some physical properties of prepared Ag NPs are investigated using  X-ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), optical absorption which used to characterize the structural, morphology, and optical properties of prepared samples. The crystallite size of Ag NPs that prepared using chitosan extract was ranged from (20.8 to 64.4 ) nm with a cube shape . UV-Vis absorption spectrum of prepared Ag NPs showed that the energy gap is 3.6 eV.  FESEM images confirmed that   particles size of prepared Ag NPs  wan ranged from 52 nm to 91 nm with spherical shape. Antibacterial activity of prepared Ag NPs was test against two type of bacteria, Gram-positive (Staphylococcus) and Gram-negative (Escherichia coils