Isolation and identification bacteriophage of E coli O157: H7 Acute Diarrhea children patients

Abstract

Bacteriophages are viral particles present everywhere bacteria can be found. Current estimates suggest that over 10^31 bacteriophages exist in the world, which is greater than the total of all living organisms and even bacteria combined. This study gathered a total of5out of 100samples comprising stool from children patient with acute diarrhea   in order to investigate if a highly effective phage alternative therapy could be developed. Four different antibiotics were tested using Kirby- Bauer Method for sensitivity tests the results indicated isolation showed high resistance (100%) for Ceftazidime, Levofloxacin, Ciprofloxacin ceftriaxone, Antibiotics. while showed Low resistance with (0%) for Meropenem. Phage antibacterial efficacy was evaluated against four strains of Escherichia coli O157:H7 expressing multidrug-resistance isolated from children suffering from diarrhea feverish illness. All tested isolates were responsive (40%) to the action of bacteriophage lysis.. Final results indicated that 60% of our study was highly active against phages in vivo, whereas 50% was sensitive for them in vitro E.coli O157:H7. This indicates that indeed bacteriophage applied as an anti E.coli O157:H7 therapy works remarkably well on the targeted strain of application gastrointestinal tract.